Factors Affecting a Routine Novobiocin Cylinder-Plate Assay

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Factors affecting the assay of hyaluronidase.

A number of different types of assay methods have been developed for the determination of hyaluronidase activity in vitro. These include the mucin clot test (1, 2), the viscosimetric assay (3-12), the turbidimetric assay (7, 13-17), and the reductimetric assay (18). Of these, the turbidimetric and viscosimetric assays have been used most widely. In the work reported here a study has been made o...

a framework for identifying and prioritizing factors affecting customers’ online shopping behavior in iran

the purpose of this study is identifying effective factors which make customers shop online in iran and investigating the importance of discovered factors in online customers’ decision. in the identifying phase, to discover the factors affecting online shopping behavior of customers in iran, the derived reference model summarizing antecedents of online shopping proposed by change et al. was us...

Factors affecting a cyanogen bromide-based assay of thiamin.

We analyzed extensively a modified thiochrome method for thiamin analysis. Acid phosphatase (EC 3.1.3.2) from potato was superior to either alpha-amylase or acid phosphatase from wheat germ as a dephosphorylating agent. Timing of cyanogen bromide exposure was important, but the assay had good precision and accuracy. The standard curve was linear from 10 to 3000 nmol/L. The within-run and betwee...

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